Biuret Reagent MSDS
Section 1: Chemical Product and Company Identification
Product Name: Biuret Reagent
Catalog Codes: SLB2656
RTECS: Not applicable.
TSCA: TSCA 8(b) inventory: Water; Sodium hydroxide
CI#: Not applicable.
Synonym: Biuret Reagent
Chemical Name: Not applicable.
Chemical Formula: Not applicable.
Section 2: Composition and Information on Ingredients
Name CAS # % by Weight
Water 7732-18-5 94.3
Sodium hydroxide 1310-73-2 5
Potassium sodium tartrate 6381-59-5 0.6
Copper sulfate pentahydrate 7758-99-8 0.15
Toxicological Data on Ingredients: Sodium hydroxide LD50: Not available. LC50: Not available. Copper sulfate
pentahydrate: ORAL (LD50): Acute: 300 mg/kg [Rat.]. DERMAL (LD50): Acute: >2000 mg/kg [Rat].
Section 3: Hazards Identification
Potential Acute Health Effects:
Very hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, . Hazardous in case of skin
contact (corrosive, permeator), of eye contact (corrosive). Liquid or spray mist may produce tissue damage
particularly on mucous membranes of eyes, mouth and respiratory tract. Skin contact may produce burns.
Inhalation of the spray mist may produce severe irritation of respiratory tract, characterized by coughing, choking,
or shortness of breath. Inflammation of the eye is characterized by redness, watering, and itching. Skin
inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.
Potential Chronic Health Effects:
CARCINOGENIC EFFECTS: Not available.
MUTAGENIC EFFECTS: Mutagenic for mammalian somatic cells. [Sodium hydroxide]. Mutagenic for
mammalian somatic cells. [Copper sulfate pentahydrate].
TERATOGENIC EFFECTS: Not available.
DEVELOPMENTAL TOXICITY: Not available.
The substance is toxic to mucous membranes.
The substance may be toxic to kidneys, liver.
Repeated or prolonged exposure to the substance can produce target organs damage. Repeated or prolonged
contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or prolonged
exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection.
Section 4: First Aid Measures
Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at
least 15 minutes. Cold water may be used. Get medical attention immediately.
In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated
clothing and shoes. Cover the irritated skin with an emollient. Cold water may be used.Wash clothing before
reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.
Serious Skin Contact:
Wash with a disinfectant soap and cover the contaminated skin with an anti-bacterial cream. Seek medical
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get
medical attention immediately.
Evacuate the victim to a safe area as soon as possible. Loosen tight clothing such as a collar, tie, belt or
waistband. If breathing is difficult, administer oxygen. If the victim is not breathing, perform mouth-to-mouth
resuscitation. Seek medical attention.
Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an
unconscious person. If large quantities of this material are swallowed, call a physician immediately. Loosen tight
clothing such as a collar, tie, belt or waistband.
Serious Ingestion: Not available.
Section 5: Fire and Explosion Data
Flammability of the Product: Non-flammable.
Auto-Ignition Temperature: Not applicable.
Flash Points: Not applicable.
Flammable Limits: Not applicable.
Products of Combustion: Not available.
Fire Hazards in Presence of Various Substances: Not applicable.
Explosion Hazards in Presence of Various Substances: Non-explosive in presence of open flames and sparks, of shocks.
Fire Fighting Media and Instructions: Not applicable.
Special Remarks on Fire Hazards: Not available.
Special Remarks on Explosion Hazards:
Sodium hydroxide reacts to form explosive products with ammonia + silver nitrate.
Benzene extract of allyl benzenesulfonate prepared from allyl alcohol, and benzene sulfonyl chloride in presence of
aquesous sodium hydroxide, under vacuum distillation, residue darkened and exploded.
Sodium Hydroxde + impure tetrahydrofuran, which can contain peroxides, can cause serious explosions.
Dry mixtures of sodium hydroxide and sodium tetrahydroborate liberate hydrogen explosively at 230-270 deg. C.
Sodium Hydroxide reacts with sodium salt of trichlorophenol + methyl alcohol + trichlorobenzene + heat to cause
an explosion. (Sodium hydroxide)
Section 6: Accidental Release Measures
Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal
container. If necessary: Neutralize the residue with a dilute solution of acetic acid.
Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not get water inside
container. Do not touch spilled material. Use water spray curtain to divert vapor drift. Prevent entry into sewers,
basements or confined areas; dike if needed. Call for assistance on disposal. Neutralize the residue with a
dilute solution of acetic acid. Be careful that the product is not present at a concentration level above TLV.
Check TLV on the MSDS and with local authorities.
Section 7: Handling and Storage
Keep locked up.. Keep container dry. Do not breathe gas/fumes/ vapor/spray. Never add water to this product.
In case of insufficient ventilation, wear suitable respiratory equipment. If you feel unwell, seek medical attention
and show the label when possible. Avoid contact with skin and eyes. Keep away from incompatibles such as
Storage: Keep container tightly closed. Keep container in a cool, well-ventilated area.
Section 8: Exposure Controls/Personal Protection
Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their
respective threshold limit value.
Face shield. Full suit. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves.
Personal Protection in Case of a Large Spill:
Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self contained breathing apparatus should be
used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist
BEFORE handling this product.
STEL: 2 (mg/m3) from ACGIH (TLV) [United States]
TWA: 2 CEIL: 2 (mg/m3) from OSHA (PEL) [United States]
CEIL: 2 (mg/m3) from NIOSH
Copper sulfate pentahydrate
TWA: 1 (mg/m3) from ACGIH (TLV) [United States] Inhalation
TWA: 0.1 (mg/m3) from OSHA (PEL) [United States] Inhalation
TWA: 1 (mg/m3) from NIOSH Inhalation3
Consult local authorities for acceptable exposure limits.
Section 9: Physical and Chemical Properties
Physical state and appearance: Liquid.
Odor: Not available.
Taste: Not available.
Molecular Weight: Not applicable.
Color: Not available.
pH (1% soln/water): Not available
Boiling Point: The lowest known value is 100°C (212°F) (Water).
Melting Point: Not available.
Critical Temperature: Not available.
Specific Gravity: Weighted average: 1.03 (Water = 1)
Vapor Pressure: The highest known value is 2.3 kPa (@ 20°C) (Water).
Vapor Density: The highest known value is 0.62 (Air = 1) (Water).
Volatility: Not available.
Odor Threshold: Not available.
Water/Oil Dist. Coeff.: Not available.
Ionicity (in Water): Not available.
Dispersion Properties: See solubility in water, methanol.
Easily soluble in cold water.
Soluble in hot water.
Partially soluble in methanol.
Section 10: Stability and Reactivity Data
Stability: The product is stable.
Instability Temperature: Not available.
Conditions of Instability: Incompatible materials
Incompatibility with various substances:
Reactive with metals.
Slightly reactive to reactive with oxidizing agents, reducing agents, acids, alkalis.
Highly corrosive in presence of aluminum.
Slightly corrosive in presence of steel, of stainless steel(304), of stainless steel(316), brass, bronze.
Non-corrosive in presence of glass, of copper.
Special Remarks on Reactivity:
Sodium hydroxide solution and octanol + diborane during a work-up of a reaction mixture of oxime and diborane in
tetrahyrofuran is very exothermic, a mild explosion being noted on one occassion.
Reactive with water, acids (mineral, non-oxidizing, e.g. hydrochloric, hydrofluoric acid, muriatic acid, phosphoric),
acids (mineral, oxidizing e.g. chromic acid, hypochlorous acid, nitric acid, sulfuric acid), acids (organic e.g. acetic
acid, benzoic acid, formic acid, methanoic acid, oxalic acid), aldehydes (e.g. acetaldehyde, acrolein, chloral
hydrate, foraldehyde), carbamates (e.g. carbanolate, carbofuran), esters (e.g. butyl acetate, ethyl acetate, propyl
formate), halogenated organics (dibromoethane, hexachlorobenzene, methyl chloride, trichloroethylene),
isocyanates (e.g. methyl isocyanate), ketones (acetone, acetophenone, MEK, MIBK), acid chlorides, strong
bases, strong oxidizing agents, strong reducing agents, flammable liquids, powdered metals and metals (i.e
aluminum, tin, zinc, hafnium, raney nickel), metals (alkali and alkaline e.g. cesium, potassium, sodium), metal
compounds (toxic e.g. berylium, lead acetate, nickel carbonyl, tetraethyl lead), mitrides (e.g. potassium nitride,
sodium nitride), nitriles (e.g. acetonitrile, methyl cyanide), nitro compounds (organic e.g. nitrobenzene,
nitromethane), acetic anhydride, chlorohydrin, chlorosulfonic acid, ethylene cyanohydrin, glyoxal, hydrosulfuric
acid, oleum, propiolactone, acylonitrile, phorosous pentoxide, chloroethanol, chloroform-methanol,
tetrahydroborate, cyanogen azide, 1,2,4,5 tetrachlorobenzene, cinnamaldehyde.
Reacts with formaldehyde hydroxide to yield formic acid, and hydrogen. (Sodium hydroxid
Special Remarks on Corrosivity: Very caustic to aluminum and other metals in presence of moisture. (Sodium hydroxide)
Polymerization: Will not occur.
Section 11: Toxicological Information
Routes of Entry: Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion.
Toxicity to Animals:
LD50: Not available.
LC50: Not available.
Chronic Effects on Humans:
MUTAGENIC EFFECTS: Mutagenic for mammalian somatic cells. [Sodium hydroxide]. Mutagenic for
mammalian somatic cells. [Copper sulfate pentahydrate].
Contains material which may cause damage to the following organs: kidneys, liver.
Other Toxic Effects on Humans:
Very hazardous in case of skin contact (irritant), of ingestion, .
Hazardous in case of skin contact (corrosive, permeator), of eye contact (corrosive), of inhalation (lung corrosive).
Special Remarks on Toxicity to Animals:
Lowest Published Lethal Dose:
LDL [Human] - Route: Oral; Dose: 1088 mg/kg (Copper sulfate pentahydrate)
Special Remarks on Chronic Effects on Humans: May affect genetic material. Investigation as a mutagen (cytogenetic
analysis) (Sodium hydroxide)
Special Remarks on other Toxic Effects on Humans:
Acute Potential Health Effects:
Skin: May be harmful if absorbed through skin. Causes severe skin irritation and burns. May cause deep
penetrating ulcers of the skin.
Eyes: Causes severe eye irritation and burns. May cause chemical conjunctivitis and corneal damage.
Inhalation: Harmful if inhaled. Causes severe irritation of the respiratory tract and mucous membranes with
coughing, burns, breathing difficulty, and possible coma. Irritation may lead the chemical pneumonitis and
pulmonary edema. Causes chemical burns to the respiratory tract and mucous membranes.
Ingestion: May be fatal if swallowed. May cause severe and permanent damage to the digestive tract. Causes
severe gastrointestinal tract irritation and burns. May cause perforation of the digestive tract. Causes severe
pain, nausea, vomiting, diarrhea, and shock. May cause corrosion and permanent destruction of the esophagus
and digestive tract. May affect metabolism, liver (jaundice), blood (Methemoglobin, hemalytic anemia), urinary
system (kidneys), behavior/nervous systems (somnolence, coma), cardiovascular system (lowering of blood
Section 12: Ecological Information
Ecotoxicity: Not available.
BOD5 and COD: Not available.
Products of Biodegradation:
Possibly hazardous short term degradation products are not likely. However, long term degradation products may
Toxicity of the Products of Biodegradation: The product itself and its products of degradation are not toxic.
Special Remarks on the Products of Biodegradation:
If released to soil, copper sulfate may leach to groundwater, be partly oxidized, or bind to humic materials, clay, or
hydrous of iron and manganese. In water, it will bind to carbonates as well as humic materials, clay and hydrous
oxides of iron and manganese. Copper is accumulated by plants and animals, but it does not appear to
biomagnify from plants to animals. This lack of biomagnification appears common with heavy metals. In air,
copper aerosols (in general) have a residence time of 2 to 10 days in an unpolluted atmosphere and 0.1 to >4 in a
polluted, urban areas. (Copper sulfate pentahydrate)
Section 13: Disposal Considerations
Waste must be disposed of in accordance with federal, state and local environmental
Section 14: Transport Information
DOT Classification: Class 8: Corrosive material
Identification: : Sodium Hydroxide Solution (Sodium hydroxide) UNNA: 1824 PG: III
Special Provisions for Transport: Not available
Section 15: Other Regulatory Information
Federal and State Regulations:
Illinois toxic substances disclosure to employee act: Sodium hydroxide
Illinois chemical safety act: Sodium hydroxide
New York release reporting list: Sodium hydroxide
Rhode Island RTK hazardous substances: Sodium hydroxide
Pennsylvania RTK: Sodium hydroxide
Minnesota: Sodium hydroxide
Massachusetts RTK: Sodium hydroxide
New Jersey: Sodium hydroxide
Louisiana spill reporting: Sodium hydroxide
TSCA 8(b) inventory: Water; Sodium hydroxide
CERCLA: Hazardous substances.: Sodium hydroxide: 1000 lbs. (453.6 kg);
Other Regulations: OSHA: Hazardous by definition of Hazard Communication Standard (29 CFR 1910.1200).
WHMIS (Canada): CLASS E: Corrosive liquid.
R34- Causes burns.
S26- In case of contact with eyes, rinse
immediately with plenty of water and seek
S36/37/39- Wear suitable protective clothing,
gloves and eye/face protection.
S45- In case of accident or if you feel unwell,
seek medical advice immediately (show the
label where possible).
S46- If swallowed, seek medical advice
immediately and show this container or label.
Health Hazard: 3
Fire Hazard: 0
National Fire Protection Association (U.S.A.):
Vapor respirator. Be sure to use an
approved/certified respirator or
equivalent. Wear appropriate respirator
when ventilation is inadequate.
Section 16: Other Information
References: Not available.
Other Special Considerations: Not available.
Created: 10/10/2005 12:52 AM
Last Updated: 11/06/2008 12:00 PM