Biuret Reagent MSDS


Section 1: Chemical Product and Company Identification

Product Name: Biuret Reagent

Catalog Codes: SLB2656

CAS#: Mixture.

RTECS: Not applicable.

TSCA: TSCA 8(b) inventory: Water; Sodium hydroxide

CI#: Not applicable.

Synonym: Biuret Reagent

Chemical Name: Not applicable.

Chemical Formula: Not applicable.


Section 2: Composition and Information on Ingredients


Name CAS # % by Weight

Water 7732-18-5 94.3

Sodium hydroxide 1310-73-2 5

Potassium sodium tartrate 6381-59-5 0.6

Copper sulfate pentahydrate 7758-99-8 0.15

Toxicological Data on Ingredients: Sodium hydroxide LD50: Not available. LC50: Not available. Copper sulfate

pentahydrate: ORAL (LD50): Acute: 300 mg/kg [Rat.]. DERMAL (LD50): Acute: >2000 mg/kg [Rat].


Section 3: Hazards Identification

Potential Acute Health Effects:

Very hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, . Hazardous in case of skin

contact (corrosive, permeator), of eye contact (corrosive). Liquid or spray mist may produce tissue damage

particularly on mucous membranes of eyes, mouth and respiratory tract. Skin contact may produce burns.

Inhalation of the spray mist may produce severe irritation of respiratory tract, characterized by coughing, choking,

or shortness of breath. Inflammation of the eye is characterized by redness, watering, and itching. Skin

inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.

Potential Chronic Health Effects:


MUTAGENIC EFFECTS: Mutagenic for mammalian somatic cells. [Sodium hydroxide]. Mutagenic for

mammalian somatic cells. [Copper sulfate pentahydrate].



The substance is toxic to mucous membranes.

The substance may be toxic to kidneys, liver.

Repeated or prolonged exposure to the substance can produce target organs damage. Repeated or prolonged

contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or prolonged

exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection.


Section 4: First Aid Measures

Eye Contact:

Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at

least 15 minutes. Cold water may be used. Get medical attention immediately.

Skin Contact:

In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated

clothing and shoes. Cover the irritated skin with an emollient. Cold water may be used.Wash clothing before

reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.

Serious Skin Contact:

Wash with a disinfectant soap and cover the contaminated skin with an anti-bacterial cream. Seek medical



If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get

medical attention immediately.

Serious Inhalation:

Evacuate the victim to a safe area as soon as possible. Loosen tight clothing such as a collar, tie, belt or

waistband. If breathing is difficult, administer oxygen. If the victim is not breathing, perform mouth-to-mouth

resuscitation. Seek medical attention.


Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an

unconscious person. If large quantities of this material are swallowed, call a physician immediately. Loosen tight

clothing such as a collar, tie, belt or waistband.

Serious Ingestion: Not available.


Section 5: Fire and Explosion Data

Flammability of the Product: Non-flammable.

Auto-Ignition Temperature: Not applicable.

Flash Points: Not applicable.

Flammable Limits: Not applicable.

Products of Combustion: Not available.

Fire Hazards in Presence of Various Substances: Not applicable.

Explosion Hazards in Presence of Various Substances: Non-explosive in presence of open flames and sparks, of shocks.

Fire Fighting Media and Instructions: Not applicable.

Special Remarks on Fire Hazards: Not available.

Special Remarks on Explosion Hazards:

Sodium hydroxide reacts to form explosive products with ammonia + silver nitrate.

Benzene extract of allyl benzenesulfonate prepared from allyl alcohol, and benzene sulfonyl chloride in presence of

aquesous sodium hydroxide, under vacuum distillation, residue darkened and exploded.

Sodium Hydroxde + impure tetrahydrofuran, which can contain peroxides, can cause serious explosions.

Dry mixtures of sodium hydroxide and sodium tetrahydroborate liberate hydrogen explosively at 230-270 deg. C.

Sodium Hydroxide reacts with sodium salt of trichlorophenol + methyl alcohol + trichlorobenzene + heat to cause

an explosion. (Sodium hydroxide)


Section 6: Accidental Release Measures

Small Spill:

Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal

container. If necessary: Neutralize the residue with a dilute solution of acetic acid.

Large Spill:

Corrosive liquid.

Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not get water inside

container. Do not touch spilled material. Use water spray curtain to divert vapor drift. Prevent entry into sewers,

basements or confined areas; dike if needed. Call for assistance on disposal. Neutralize the residue with a

dilute solution of acetic acid. Be careful that the product is not present at a concentration level above TLV.

Check TLV on the MSDS and with local authorities.


Section 7: Handling and Storage


Keep locked up.. Keep container dry. Do not breathe gas/fumes/ vapor/spray. Never add water to this product.

In case of insufficient ventilation, wear suitable respiratory equipment. If you feel unwell, seek medical attention

and show the label when possible. Avoid contact with skin and eyes. Keep away from incompatibles such as


Storage: Keep container tightly closed. Keep container in a cool, well-ventilated area.


Section 8: Exposure Controls/Personal Protection

Engineering Controls:

Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their

respective threshold limit value.

Personal Protection:

Face shield. Full suit. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves.


Personal Protection in Case of a Large Spill:

Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self contained breathing apparatus should be

used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist

BEFORE handling this product.

Exposure Limits:

Sodium hydroxide

STEL: 2 (mg/m3) from ACGIH (TLV) [United States]

TWA: 2 CEIL: 2 (mg/m3) from OSHA (PEL) [United States]

CEIL: 2 (mg/m3) from NIOSH

Copper sulfate pentahydrate

TWA: 1 (mg/m3) from ACGIH (TLV) [United States] Inhalation

TWA: 0.1 (mg/m3) from OSHA (PEL) [United States] Inhalation

TWA: 1 (mg/m3) from NIOSH Inhalation3

Consult local authorities for acceptable exposure limits.


Section 9: Physical and Chemical Properties

Physical state and appearance: Liquid.

Odor: Not available.

Taste: Not available.

Molecular Weight: Not applicable.

Color: Not available.

pH (1% soln/water): Not available

Boiling Point: The lowest known value is 100C (212F) (Water).

Melting Point: Not available.

Critical Temperature: Not available.

Specific Gravity: Weighted average: 1.03 (Water = 1)

Vapor Pressure: The highest known value is 2.3 kPa (@ 20C) (Water).

Vapor Density: The highest known value is 0.62 (Air = 1) (Water).

Volatility: Not available.

Odor Threshold: Not available.

Water/Oil Dist. Coeff.: Not available.

Ionicity (in Water): Not available.

Dispersion Properties: See solubility in water, methanol.


Easily soluble in cold water.

Soluble in hot water.

Partially soluble in methanol.


Section 10: Stability and Reactivity Data

Stability: The product is stable.

Instability Temperature: Not available.

Conditions of Instability: Incompatible materials

Incompatibility with various substances:

Reactive with metals.

Slightly reactive to reactive with oxidizing agents, reducing agents, acids, alkalis.


Highly corrosive in presence of aluminum.

Slightly corrosive in presence of steel, of stainless steel(304), of stainless steel(316), brass, bronze.

Non-corrosive in presence of glass, of copper.

Special Remarks on Reactivity:

Sodium hydroxide solution and octanol + diborane during a work-up of a reaction mixture of oxime and diborane in

tetrahyrofuran is very exothermic, a mild explosion being noted on one occassion.

Reactive with water, acids (mineral, non-oxidizing, e.g. hydrochloric, hydrofluoric acid, muriatic acid, phosphoric),

acids (mineral, oxidizing e.g. chromic acid, hypochlorous acid, nitric acid, sulfuric acid), acids (organic e.g. acetic

acid, benzoic acid, formic acid, methanoic acid, oxalic acid), aldehydes (e.g. acetaldehyde, acrolein, chloral

hydrate, foraldehyde), carbamates (e.g. carbanolate, carbofuran), esters (e.g. butyl acetate, ethyl acetate, propyl

formate), halogenated organics (dibromoethane, hexachlorobenzene, methyl chloride, trichloroethylene),

isocyanates (e.g. methyl isocyanate), ketones (acetone, acetophenone, MEK, MIBK), acid chlorides, strong

bases, strong oxidizing agents, strong reducing agents, flammable liquids, powdered metals and metals (i.e

aluminum, tin, zinc, hafnium, raney nickel), metals (alkali and alkaline e.g. cesium, potassium, sodium), metal

compounds (toxic e.g. berylium, lead acetate, nickel carbonyl, tetraethyl lead), mitrides (e.g. potassium nitride,

sodium nitride), nitriles (e.g. acetonitrile, methyl cyanide), nitro compounds (organic e.g. nitrobenzene,

nitromethane), acetic anhydride, chlorohydrin, chlorosulfonic acid, ethylene cyanohydrin, glyoxal, hydrosulfuric

acid, oleum, propiolactone, acylonitrile, phorosous pentoxide, chloroethanol, chloroform-methanol,

tetrahydroborate, cyanogen azide, 1,2,4,5 tetrachlorobenzene, cinnamaldehyde.

Reacts with formaldehyde hydroxide to yield formic acid, and hydrogen. (Sodium hydroxid

Special Remarks on Corrosivity: Very caustic to aluminum and other metals in presence of moisture. (Sodium hydroxide)

Polymerization: Will not occur.


Section 11: Toxicological Information

Routes of Entry: Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion.

Toxicity to Animals:

LD50: Not available.

LC50: Not available.

Chronic Effects on Humans:

MUTAGENIC EFFECTS: Mutagenic for mammalian somatic cells. [Sodium hydroxide]. Mutagenic for

mammalian somatic cells. [Copper sulfate pentahydrate].

Contains material which may cause damage to the following organs: kidneys, liver.

Other Toxic Effects on Humans:

Very hazardous in case of skin contact (irritant), of ingestion, .

Hazardous in case of skin contact (corrosive, permeator), of eye contact (corrosive), of inhalation (lung corrosive).

Special Remarks on Toxicity to Animals:

Lowest Published Lethal Dose:

LDL [Human] - Route: Oral; Dose: 1088 mg/kg (Copper sulfate pentahydrate)

Special Remarks on Chronic Effects on Humans: May affect genetic material. Investigation as a mutagen (cytogenetic

analysis) (Sodium hydroxide)

Special Remarks on other Toxic Effects on Humans:

Acute Potential Health Effects:

Skin: May be harmful if absorbed through skin. Causes severe skin irritation and burns. May cause deep

penetrating ulcers of the skin.

Eyes: Causes severe eye irritation and burns. May cause chemical conjunctivitis and corneal damage.

Inhalation: Harmful if inhaled. Causes severe irritation of the respiratory tract and mucous membranes with

coughing, burns, breathing difficulty, and possible coma. Irritation may lead the chemical pneumonitis and

pulmonary edema. Causes chemical burns to the respiratory tract and mucous membranes.

Ingestion: May be fatal if swallowed. May cause severe and permanent damage to the digestive tract. Causes

severe gastrointestinal tract irritation and burns. May cause perforation of the digestive tract. Causes severe

pain, nausea, vomiting, diarrhea, and shock. May cause corrosion and permanent destruction of the esophagus

and digestive tract. May affect metabolism, liver (jaundice), blood (Methemoglobin, hemalytic anemia), urinary

system (kidneys), behavior/nervous systems (somnolence, coma), cardiovascular system (lowering of blood



Section 12: Ecological Information

Ecotoxicity: Not available.

BOD5 and COD: Not available.

Products of Biodegradation:

Possibly hazardous short term degradation products are not likely. However, long term degradation products may


Toxicity of the Products of Biodegradation: The product itself and its products of degradation are not toxic.

Special Remarks on the Products of Biodegradation:

If released to soil, copper sulfate may leach to groundwater, be partly oxidized, or bind to humic materials, clay, or

hydrous of iron and manganese. In water, it will bind to carbonates as well as humic materials, clay and hydrous

oxides of iron and manganese. Copper is accumulated by plants and animals, but it does not appear to

biomagnify from plants to animals. This lack of biomagnification appears common with heavy metals. In air,

copper aerosols (in general) have a residence time of 2 to 10 days in an unpolluted atmosphere and 0.1 to >4 in a

polluted, urban areas. (Copper sulfate pentahydrate)


Section 13: Disposal Considerations

Waste Disposal:

Waste must be disposed of in accordance with federal, state and local environmental

control regulations.


Section 14: Transport Information

DOT Classification: Class 8: Corrosive material

Identification: : Sodium Hydroxide Solution (Sodium hydroxide) UNNA: 1824 PG: III

Special Provisions for Transport: Not available


Section 15: Other Regulatory Information

Federal and State Regulations:

Illinois toxic substances disclosure to employee act: Sodium hydroxide

Illinois chemical safety act: Sodium hydroxide

New York release reporting list: Sodium hydroxide

Rhode Island RTK hazardous substances: Sodium hydroxide

Pennsylvania RTK: Sodium hydroxide

Minnesota: Sodium hydroxide

Massachusetts RTK: Sodium hydroxide

New Jersey: Sodium hydroxide

Louisiana spill reporting: Sodium hydroxide

TSCA 8(b) inventory: Water; Sodium hydroxide

CERCLA: Hazardous substances.: Sodium hydroxide: 1000 lbs. (453.6 kg);

Other Regulations: OSHA: Hazardous by definition of Hazard Communication Standard (29 CFR 1910.1200).

Other Classifications:

WHMIS (Canada): CLASS E: Corrosive liquid.


R34- Causes burns.

S26- In case of contact with eyes, rinse

immediately with plenty of water and seek

medical advice.

S36/37/39- Wear suitable protective clothing,

gloves and eye/face protection.

S45- In case of accident or if you feel unwell,

seek medical advice immediately (show the

label where possible).

S46- If swallowed, seek medical advice

immediately and show this container or label.

HMIS (U.S.A.):

Health Hazard: 3

Fire Hazard: 0

Reactivity: 0

Personal Protection:

National Fire Protection Association (U.S.A.):

Health: 2

Flammability: 0

Reactivity: 1

Specific hazard:

Protective Equipment:


Full suit.

Vapor respirator. Be sure to use an

approved/certified respirator or

equivalent. Wear appropriate respirator

when ventilation is inadequate.

Face shield.


Section 16: Other Information

References: Not available.

Other Special Considerations: Not available.

Created: 10/10/2005 12:52 AM

Last Updated: 11/06/2008 12:00 PM